Journal: Molecular Medicine Reports

Article Title: Heat shock protein 72 suppresses apoptosis by increasing the stability of X-linked inhibitor of apoptosis protein in renal ischemia/reperfusion injury

doi: 10.3892/mmr.2014.2939

Figure Lengend Snippet: Effects of GGA on renal tubular cell apoptosis. (A) Representative images of TUNEL in different groups as indicated (magnification, ×400). (B) Morphometric analysis of the number of TUNEL-positive cells per high-power field in microscopy. (C) Expression of XIAP and HSP72 in the kidney was examined by immunoblot, β-actin served as a loading control. (D) Graphic representation of XIAP protein levels in different groups, as indicated following normalization with β-actin content. Data in B and D are expressed as the mean ± standard error of the mean; * P<0.01 versus sham-surgery rats; # P<0.05 versus vehicle-treated rats. GGA, geranylgeranylacetone; XIAP, X-linked inhibitor of apoptosis protein; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; HSP72, heat shock protein 72.

Article Snippet: Apoptosis was quantified in histological sections using a commercially available TACS TdT-Fluor In Situ Apoptosis Detection kit (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions and our previous study ( ).

Techniques: TUNEL Assay, Microscopy, Expressing, Western Blot, End Labeling

Journal: Molecular Medicine Reports

Article Title: Heat shock protein 72 suppresses apoptosis by increasing the stability of X-linked inhibitor of apoptosis protein in renal ischemia/reperfusion injury

doi: 10.3892/mmr.2014.2939

Figure Lengend Snippet: Effect of HSP72 on the expression of apoptotic proteins. (A) XIAP and pro-caspase 3 were assessed by immunoblot following overexpressing HSP72. Empty vector served as negative control; (B) Quantitative determination of the relative abundance of XIAP among different groups. (C) Quantitative determination of the relative abundance of pro-caspase 3 among different groups. Data in B and C are expressed as the mean ± standard error of the mean of three experiments. * P<0.01 versus empty vector control. XIAP, X-linked inhibitor of apoptosis protein; ATP, adenosine triphosphate; HSP72, heat shock protein 72.

Article Snippet: Apoptosis was quantified in histological sections using a commercially available TACS TdT-Fluor In Situ Apoptosis Detection kit (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions and our previous study ( ).

Techniques: Expressing, Western Blot, Plasmid Preparation, Negative Control

Journal: Molecular Medicine Reports

Article Title: Heat shock protein 72 suppresses apoptosis by increasing the stability of X-linked inhibitor of apoptosis protein in renal ischemia/reperfusion injury

doi: 10.3892/mmr.2014.2939

Figure Lengend Snippet: Interaction between HSP72 and XIAP. XIAP was immunoprecipitated from whole Human kidney-2 cell lysates among different groups using a rabbit polyclonal anti-XIAP antibody. HSP72 content was assessed using an anti-HSP72 antibody (upper panel). Immunoblot analysis was used to localize XIAP in cell lysates (lower panel). XIAP, X-linked inhibitor of apoptosis protein; ATP, adenosine triphosphate; IP, immunoprecipitation; IB, immunoblotting; HSP72, heat shock protein 72.

Article Snippet: Apoptosis was quantified in histological sections using a commercially available TACS TdT-Fluor In Situ Apoptosis Detection kit (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions and our previous study ( ).

Techniques: Immunoprecipitation, Western Blot

Journal: Cardiovascular research

Article Title: Deficiency of senescence marker protein 30 exacerbates angiotensin II-induced cardiac remodelling.

doi: 10.1093/cvr/cvt122

Figure Lengend Snippet: Figure 4 Apoptosis and apoptotic signalling pathways in WT and SMP30-KO mice after Ang II infusion. (A) Upper panels show representative images of TUNEL staining of left ventricular tissue sections. Lower bar graph showsthe per cent of TUNEL-positive nuclei. (B) Activation of caspase-3 wasexamined by western blotting with anti-activated-caspase-3 antibody using myocardial samples. Expression levels of activated-caspase-3 were normalized by b-actin. (C) Expressions of Bax and Bcl-2 were analysed by western blotting. The Bax to Bcl-2 ratio was calculated and presented in the bar graph. (D) Phosphor- ylation activity of SAPK/JNK. Expressions of phosphorylated and total SAPK/JNK were analysed by western blotting. Relative expression levels of phos- phorylated SAPK/JNK (P-SAPK/JNK) were expressed in relation to those of SAPK/JNK. Results are mean + SD from 6 to 10 mice in each group. *P , 0.01 vs. control in the same strain mice; †P , 0.01 vs. Ang II-infused WT mice.

Article Snippet: The signals from immunoreactive bands were visualized by an Amersham ECL system (Amersham Pharmacia Biotech UK Ltd., Buckinghamshire, UK) and quantified using densitometric analysis. nadolu U niversity on M ay 13, 2014 http://cardiovascres.oxfordjournals.org/ D ow nloaded from 2.9 In vivo terminal deoxynucleotidyl transferase-mediated dUTP nick end- labellingassay Apoptosis was detected by the terminal deoxynucleotidyl transferasemediated dUTP nick end-labelling (TUNEL) method (CardioTACS In Situ Apoptosis Detection Kit, Trevigen, Inc., Gaithersburg, MD, USA) according to the manufacturer’s instructions.

Techniques: TUNEL Assay, Staining, Activation Assay, Western Blot, Expressing, Activity Assay, Control